Application of Species-Specific Polymerase Chain Reaction and Cytocrome b Gene for Different Meat Species Authentication
نویسندگان
چکیده
منابع مشابه
Identification of Meat Species by Polymerase Chain Reaction ( PCR ) Technique
The origin of horse, dog, cat, bovine, sheep, porcine, and goat meat was determined by the polymerase chain reaction (PCR) technique, using species-specific primers. Test mixtures of meat were prepared by adding 5%, 2.5%, 1%, 0.5%, and 0.1% levels of pork, horse, cat, or dog meat to beef, sheep, and goat meat. Samples taken from those combinations were analyzed by PCR for species determination....
متن کاملApplication of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2
متن کامل
Comparison of Two Multiplex PCR Systems for Meat Species Authentication
Background: Meat species adulteration has become a problem of concern. This study aimed to compare two previously published multiplex Polymerase Chain Reaction (PCR) methods for meat species authentication. Methods: The primers used in the first multiplex PCR involved species-specific reverse primer for sheep, goat, cattle, pig, and donkey with universal forward primer. In the second multiple...
متن کاملSpecies Specific DNA Profiling Mycobacterial Genomes Using Polymerase Chain Reaction with Single Universal Primer (UP-PCR)
Three tuberculous, twenty-one non-tuberculous mycobacterial (NTM) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of DNA fragments in a polymerase chain reaction with single universal primer (UP-PCR). A rather wide limit of tolerance for variations in procedure of PCR mixture preparation and thermocycling parameters was found. There...
متن کاملConventional multiplex polymerase chain reaction ( PCR ) versus real - time PCR for species - specific meat
Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the su...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2007
ISSN: 1682-296X
DOI: 10.3923/biotech.2007.426.430